Timing of the G1/S transition in tobacco pollen vegetative cells as a primary step towards androgenesis in vitro

摘要: Mid-bicellular pollen vegetative cells in tobacco escape from G1 arrest and proceed to the G1/S transition towards androgenesis within 1 day under glutamine starvation conditions vitro. In Nicotiana tabacum culture system, immature grains at mid-bicellular stage can mature presence of glutamine; however, if is absent, they deviate their native cell fate a few days. The glutamine-starved cannot undergo maturation, even when supplied with later. Instead, division slowly 10 days medium containing appropriate nutrients. During period, ought into S phase as primary step androgenesis. However, this event has not been experimentally confirmed. Here, we demonstrated that proceeded approximately 15–36 h after start culture. These results were obtained by analyzing transgenic possessing fusion gene encoding nuclear-localizing GFP control an E2F motif-containing promoter isolated one DNA replication licensing factors. Observations using 5-ethynyl-2′-deoxyuridine labeling detection technique uncovered was soon followed phase. hallmarks undergoing dedifferentiation give us new insights upstream events causing also provide novel strategy increase frequency androgenic response other species, including recalcitrants.