The immune response of the brown trout Salmo trutta to lipopolysaccharide

摘要: Brown trout produced high molecular weight, thermostable, dithiothreitol sensitive, non-precipitating, complement-fixing antibodies and agglutinins to lipopolysaccharides after intramuscular injection with adjuvant. Antibodies were first detected on Day 14 reached maximum titres 56 63 days when a single was given. When either second or third administered occurred 34 40 the injection. After each increased significantly, protein concentration of sera significantly decreased. In cellulose acetate electrophoresis experiments those bands which migrated in β- γ-globulin regions increased. Antibody-secreting antigen-binding cells Days 8 4 respectively maxima between 16 18. The number per 106 lymphoid higher spleen than kidney.