GSH Activated Biotin-tagged Near-Infrared Probe for Efficient Cancer Imaging.
作者： Ruiying Guo , Feng Huang , Bo Zhang , Youyou Yan , Jinxin Che
DOI: 10.7150/THNO.32742
关键词:
摘要: Tumor imaging tools with high specificity and sensitivity are needed to aid the boundary recognition in solid tumor diagnosis surgical resection. In this study, we developed a near infra-red (NIR) probe (P6) for vitro/in vivo on basis of dual strategy cancer cell targeting stimulus-dependent activation. The selective capacity towards cells P6 was thoroughly investigated, potential mechanisms endocytosis were preliminary explored. Methods: GSH-activated biotin labelled NIR designed, synthesized characterized. GSH responsive properties systematically illustrated through UV-vis, fluorescent tests LC-MS analysis. vitro collected various living lines (i.e. SW480, HGC-27, H460, BxPC-3, KHOS) normal BEAS-2B, HLF-1, THP1) under confocal laser scanning microscopy. Probe further applied image primary human which freshly isolated from peritoneal carcinoma rectal patients. Serial sections tissues sent H&E (hematoxylin-eosin) staining imaging. Live photoacoustic used investigate both HGC-27 KHOS xenograft model. Results: could be recognized transported into by specific receptors efficiently triggered release fluorophore 4. fact, cellular uptake partially blocked addition free biotin. Furthermore, lines, as well cells, exhibiting ten-fold increase fluorescence intensity over cells. dissected tissues, distinguished cancerous area microscopy, exact same indicated staining. We also found that exhibited superior selectivity against local injection. Conclusion: dual-modal enhanced environmental stimulus fluorescence. Our provided novel insight development potentially image-guided possibly diagnosis.
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