Culture of shoot tips from adventitious shoots can eradicate Apple stem pitting virus but fails in Apple stem grooving virus

摘要: This study attempted to eradicate Apple stem pitting virus (ASPV) and grooving (ASGV) from ‘Gala’ apple using shoot tips of adventitious shoots derived leaf segments. Leaf segments were excised in vitro 4-week-old stock cultured on regeneration medium containing 9.1 µM thidiazuron 0.25 indole-3-butyric acid (IBA) induce formation. Shoot different size developmental stage tip culture 1.1 6-benzyladenine 0.05 IBA. Results showed did not influence survival rate, but significantly affected regrowth rate ASPV-free frequency. rates increased 10 15 % 0.3 mm two primordia (LP) after 2–3 weeks regeneration, 53–55 those three LP 3–4 weeks. The highest (82 %) was obtained 0.4 four 4 frequencies (95–100 high 0.2–0.4 2–4 weeks, low (20 None the regenerated ASGV-free, regardless at which excised. Histological studies localization provided explanations varying eradication that stages. protocol described here characteristic frequency ASPV efficiency production used for eradication, thus may have potential applications other plant species.