A Biosensor for Aromatic Aldehydes Comprising the Mediator Dependent PaoABC‐Aldehyde Oxidoreductase
作者： Artavazd Badalyan , Meina Neumann-Schaal , Silke Leimkühler , Ulla Wollenberger
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摘要: A novel aldehyde oxidoreductase (PaoABC) from Escherichia coli was utilized for the development of an oxygen insensitive biosensor benzaldehyde. The enzyme immobilized in polyvinyl alcohol and currents were measured oxidation with different one two electron mediators highest sensitivity benzaldehyde presence hexacyanoferrate(III). optimized respect to mediator concentration, loading pH using potassium linear measuring range is between 0.5200 mu M In correspondence substrate selectivity solution revealed a preference aromatic aldehydes less effective conversion aliphatic aldehydes. independent, which particularly attractive feature application. can be applied detect contaminations solvents such as benzyl alcohol, where traces down 0.0042?% detected.
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