Intranasal Delivery of Recombinant NT4-NAP/AAV Exerts Potential Antidepressant Effect
作者： Xian-Cang Ma , Zheng Chu , Xiao-Ling Zhang , Wen-Hui Jiang , Min Jia
DOI: 10.1007/S11064-016-1841-0
关键词:
摘要: The present study was designed to construct a recombinant adeno-associated virus (rAAV) which can express NAP in the brain and examine whether this produce antidepressant effects on C57 BL/6 mice that had been subjected open field test forced swimming test, via nose-to-brain pathway. When plasmid pGEM-T Easy/NT4-NAP digested by EcoRI, 297 bp fragments be obtained NT4-NAP sequence consistent with confirmed DNA sequencing. pSSCMV/NT4-NAP is visible. Immunohistochemical staining of fibroblasts revealed expression detected NT4-NAP/AAV group. Intranasal delivery significantly reduced immobility time when FST performed after 1 day from last administration. observed could not attributed non-specific increases activity since intranasal did alter behavior during test. results indicated AAV vector cells successfully constructed may potential target for therapeutic action treatment.
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