Label-free quantitative proteomics unravels the importance of RNA processing in glioma malignancy
作者： Baibin Bi , Feng Li , Jisheng Guo , Cuiling Li , Ruirui Jing
DOI: 10.1016/J.NEUROSCIENCE.2017.03.023
关键词:
摘要: Glioma, one of the most common cancers in human, is classified to different grades according degrees malignancy. Glioblastoma (GBM) known be malignant (Grade IV) whereas low-grade astrocytoma (LGA, Grade II) relatively benign. The mechanism underlying pathogenesis and progression glioma malignancy remains unclear. Here we report a quantitative proteomic study elucidate differences between GBM LGA using liquid chromatography tandem mass spectrometry followed by label-free quantification. A total 136 proteins were differentially expressed for at least five folds comparison with LGA. Ontological analysis revealed close correlation GBM-associated RNA processing. Interaction network indicated that processing linked crucial signaling transduction modulators including epidermal growth factor receptor (EGFR), signal transducer activator transcription 1 (STAT1), mitogen-activated protein kinase (MAPK1), which further connected important neuronal structural integrity, development functions. Upregulation 40S ribosomal S5 (RPS5), Ferritin Heavy chain (FTH1) STAT1, downregulation tenascin R (TNR) validated as representatives immune assays. In summary, panel centered RNA-processing may become novel biomarkers help mechanism.
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