Pericentriolar material analyses in normal esophageal mucosa, Barrett's metaplasia and adenocarcinoma.

摘要: Barrett's esophagus metaplasia is a pre-cancerous condition caused by chronic esophagitis. Chromosomal instability (CIN) common in cells: therefore, we investigated the possible presence of centrosomal aberrations (a main cause CIN) protein immunostaining paraffined esophageal samples patients who developed adenocarcinoma. In most (55%) patients, alterations pericentriolar material (PCM) signals were evident and consistently marked transition between normal epithelium to metaplasia. The could even be found adjacent native squamous epithelium, mucosa submucosal gland cells, as well basal/epibasal layers duct, which are regions hosting stem progenitor cells. These findings strongly support hypothesis that three histotypes (one being pathological) can have progenitor. Surprisingly, PCM defective signal eventually decreased with neoplastic progression, possibly enhance genome stability advanced cancer Importantly, altered their apparent evolution successive histopathological steps correlated adenocarcinoma aggressiveness, suggesting prognostic marker for tumor relapse. Extending our observations prospective study might help development new prevention protocols patients.