Neural Influence on the Extracellular Matrix During Blastema Formation

摘要: By investigating reinnervation of denervated/amputated limbs urodele (Ambystoma mexicanum) larvae, it is shown that nerves affect hyaluronate accumulation in the extracellular matrix as well mitotic activity cells distal, dedifferentiated regions limb stump. Accumulation increased two-fold and stimulated four-fold over seen immediately prior to reappearance nerve fibers distal areas. Formation a hyaluronate-rich accompanied by enlargement intercellular space decrease cell density, which occur simultaneously with appearance morphologically recognizable blastema on tip The increase proliferative includes entered cycle response amputation injury incorporated 3H-thymidine five days later. These results indicate promote formation growth both stimulation division enhancing production large component important for expansion tissue volume. Work others cultured mesenchymal purified factors suggests such stimulate cycling part single mitogenic effect.