Conductive architecture of Fe2O3 microspheres/self-doped polyaniline nanofibers on carbon ionic liquid electrode for impedance sensing of DNA hybridization.
作者： Wei Zhang , Tao Yang , Xiao Li , Debao Wang , Kui Jiao
DOI: 10.1016/J.BIOS.2009.07.032
关键词:
摘要: Abstract A novel architecture was designed by combining the strong adsorption ability of Fe 2 O 3 microspheres to DNA probes and excellent conductivity self-doped polyaniline (SPAN) nanofibers (copolymer aniline m -aminobenzenesulfonic acid) on carbon ionic liquid electrode (CILE) for electrochemical impedance sensing immobilization hybridization DNA. The formed conductive /SPAN membrane CILE characterized cyclic voltammetry (CV) spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− as indicator. probe surface sensitivity recognition were dramatically enhanced due unique synergistic effect microspheres, SPAN liquid. events monitored with a label-free EIS strategy. Under optimal conditions, dynamic range this biosensor detecting sequence-specific phosphoenolpyruvate carboxylase (PEPCase) gene from transgenically modified rape 1.0 × 10 −13 −7 mol/L, detection limit 2.1 × 10 −14 mol/L. This work suggested simple strategy prepare interface opened way application in biosensing.
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sci-hub.se 参考文章(37)
Petra Schrenkhammer, Otto S. Wolfbeis, Fully reversible optical biosensors for uric acid using oxygen transduction. Biosensors and Bioelectronics. ,vol. 24, pp. 994- 999 ,(2008) , 10.1016/J.BIOS.2008.08.007
Fengxian Gao, Ruo Yuan, Yaqin Chai, Shihong Chen, Shurui Cao, Mingyu Tang, Amperometric hydrogen peroxide biosensor based on the immobilization of HRP on nano-Au/Thi/poly (p-aminobenzene sulfonic acid)-modified glassy carbon electrode. Journal of Biochemical and Biophysical Methods. ,vol. 70, pp. 407- 413 ,(2007) , 10.1016/J.JBBM.2006.09.007
Fei Xiao, Faqiong Zhao, Jiangwen Li, Liqin Liu, Baizhao Zeng, Characterization of hydrophobic ionic liquid-carbon nanotubes–gold nanoparticles composite film coated electrode and the simultaneous voltammetric determination of guanine and adenine Electrochimica Acta. ,vol. 53, pp. 7781- 7788 ,(2008) , 10.1016/J.ELECTACTA.2008.05.053
Nirmal Prabhakar, Kavita Arora, Harpal Singh, Bansi D. Malhotra, Polyaniline Based Nucleic Acid Sensor Journal of Physical Chemistry B. ,vol. 112, pp. 4808- 4816 ,(2008) , 10.1021/JP711853Q
Yuxiao Cheng, Yajun Liu, Jingjing Huang, Kang Li, Yuezhong Xian, Wen Zhang, Litong Jin, Amperometric tyrosinase biosensor based on Fe3O4 nanoparticles-coated carbon nanotubes nanocomposite for rapid detection of coliforms Electrochimica Acta. ,vol. 54, pp. 2588- 2594 ,(2009) , 10.1016/J.ELECTACTA.2008.10.072
Wei Zhang, Tao Yang, Xuming Zhuang, Zhiyan Guo, Kui Jiao, An ionic liquid supported CeO2 nanoshuttles-carbon nanotubes composite as a platform for impedance DNA hybridization sensing. Biosensors and Bioelectronics. ,vol. 24, pp. 2417- 2422 ,(2009) , 10.1016/J.BIOS.2008.12.024
Dianping Tang, Ruo Yuan, Yaqin Chai, Ultrasensitive electrochemical immunosensor for clinical immunoassay using thionine-doped magnetic gold nanospheres as labels and horseradish peroxidase as enhancer. Analytical Chemistry. ,vol. 80, pp. 1582- 1588 ,(2008) , 10.1021/AC702217M
Mustafa M. Musameh, Roohollah Torabi Kachoosangi, Richard G. Compton, Enhanced stability and sensitivity of ionic liquid-carbon paste electrodes at elevated temperatures. Analyst. ,vol. 133, pp. 133- 138 ,(2008) , 10.1039/B713071F
Yanli Zhang, Ying Wang, Haibo Wang, Jian-Hui Jiang, Guo-Li Shen, Ru-Qin Yu, Jinghong Li, Electrochemical DNA biosensor based on the proximity-dependent surface hybridization assay. Analytical Chemistry. ,vol. 81, pp. 1982- 1987 ,(2009) , 10.1021/AC802512D
Jinghua Chen, Jing Zhang, Kun Wang, Xinhua Lin, Liying Huang, Guonan Chen, Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene Using Locked Nucleic Acids on 4-Aminobenzenesulfonic Acid-Modified Glassy Carbon Electrode Analytical Chemistry. ,vol. 80, pp. 8028- 8034 ,(2008) , 10.1021/AC801040E