Microtubule and chromatin configurations during rhesus intracytoplasmic sperm injection: successes and failures.

摘要: Intracytoplasmic sperm injection (ICSI) was performed on rhesus monkey oocytes, and the resultant microtubule DNA configurations were imaged by laser-scanning confocal microscopy. In addition, polyspermic oocytes fertilized ICSI examined transmission electron microscopy (TEM). Successful fertilization revealed similar to those observed during in vitro of human including aster formation, pronuclei decondensation, spindle cell division. Several abnormalities, however, also observed: 1) inability complete meiosis; 2) undergo male or female pronucleus formation; 3) separation tail from nucleus; 4) premature chromosome condensation with formation a paternal meiotic spindle; 5) multiple (karyomeres) decondensation. TEM analysis that can decondensation presence an intact acrosome at least 18 h after injection. These results demonstrate utility pre-clinical applications as well endangered species. However, different types failures here indicate although may be readily accepted means many clinics, we should further characterize cellular genetic abnormalities associated both nonhuman primates.