Changes in ribosomal protein and ribosomal RNA synthesis during rat intestinal differentiation.

摘要: Subtraction hybridization studies, used to identify genes involved in the control of enterocyte proliferation and/or differentiation, allowed detection a clone shown have homologies with rat, chicken, and human acidic ribosomal phosphoprotein P1. Since increases P1 transcript been associated intestinal malignancy, we explored relationship other proteins normal differentiation. Male rats were prepare enterocytes as isolated cell fractions representative crypt villus axis Total RNA was extracted from pooled evaluated for mRNA rRNA steady-state levels. Nuclei prepared enterocytes, nuclear runoff studies performed estimate rates nascent transcription. The complementary DNA library detected 650 base pairs which showed approximately 8-fold greater levels than cells. Similar specificity also noted mRNAs coding elongation factor EF-12 P0, P1, S6 (using clones Y-L. Chan I. G. Wool). In contrast, 28S did not differ between crypt, indicating that content had remained constant. situ confirmed predominant localization mRNA. Nascent transcription rate proportion newly synthesized total same suggesting lower may be due increased degradation.(ABSTRACT TRUNCATED AT 250 WORDS)