Exposure of monocytes to heat shock does not increase class II expression but modulates antigen-dependent T cell responses

作者: Ewa Mariéthoz , Fabienne Tacchini-Cottler , Muriel Jacquier-Sarlln , Fiona Sinclair , Barbara S. Polia

DOI: 10.1093/INTIMM/6.6.925

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摘要: Expression of heat shock (HS) proteins (HSP) increases after exposure to elevated temperatures or other types injury, such as oxidative injury. Because their function 'molecular chaperones', HSP are suggested participate in antigen processing and presentation. We have previously reported that HS modulates presentation a human EBV-transformed B cell line. Here we investigated the effects on MHC class II expression by monocytes. Monocytes were isolated from peripheral blood normal volunteers, purified adherence, then exposed ranging 37 45 degrees C for 20 min, allowed recover 2 h at used immunofluorescence presenting cells autologous heterologous lymphocyte proliferation assays. No increase was detected assessed flow cytometry. (3 x 10(4)) lymphocytes (1 10(5)) co-cultured 5 days presence several antigens [diphtheria toxoid, tetanus toxoid peptide derivative (PPD)] labeled with 1 microCi [3H]thymidine 16 h. Pre-exposure (44 C) significantly (P < 0.001) increased T responses diphtheria whereas effect (tetanus PPD) not significant. did nor induced non-processed superantigens staphylococcal enterotoxin B. The inhibited actinomycin thus appeared dependent upon synthesis. HSP-mediated may potentiate ongoing immune response inflammatory sites.

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