A comparison of cervical histopathology variability using whole slide digitized images versus glass slides: Experience with a statewide registry

摘要: Summary Whole slide imaging is increasingly used for primary and consultative diagnoses, teaching, telepathology, sharing, archiving. We compared pathologist evaluations of glass slides corresponding digitized images within the context a statewide surveillance effort. Cervical specimens collected by New Mexico HPV Pap Registry research program targeted cases diagnosed between 2006 2010. Two samples 250 each were with ScanScope XT (Aperio, Vista, CA) microscope reviewed Aperio ImageScope reader. (1) A "random set" had distribution community diagnoses: 70% from cervical intraepithelial neoplasia grade 2 or higher, 20% 1 10% negative cases. (2) "discrepant was represented difficult where study pathologists initially disagreed. Within regular workflow Registry, 3 read to times without knowledge clinical history, previous readings sampling scheme. Pathologists also image twice. For within- between-reader comparisons we calculated unweighted κ statistics asymmetry χ tests. Across all comparisons, yielded similar results. random set, almost within-reader Kappa values ranged 0.7 0.8 0.6 0.7, respectively. discrepant most 0.4 0.6. As diagnostic terminology changes, may need re-read histopathology compare disease trends over time, eg, before/after introduction human papillomavirus vaccination. Diagnosis differed little images.