Exploitation of Surface Molecules for Separation of Cells from Mosaic Livers

摘要: Publisher Summary This chapter focuses on the exploitation of surface molecules for separation cells from mosaic livers. The liver parenchyma is a biochemically tissue. natural mosaicism has been exploited to demonstrate clonal nature early hyperplastic lesions arising during chemically induced carcinogenesis in mice. Natural employed an analogous manner past clonality some human tumors. Two immunological approaches purification donor-origin hepatocytes genotypic livers, differential hepatocyte rosetting, and complement-mediated cytolysis have provided 4- 11-fold enrichments hepatocytes. A panning technique suitable host-origin combination these cell strategies should make possible preparative scale donor rat In principle, techniques be applicable other tissues which developmental or pathogenic cellular lineages must elucidated. positive immunoselective contrast two negative selection described as cells.