Incidence and mechanism of ciprofloxacin resistance in Campylobacter spp. isolated from commercial poultry flocks in the United Kingdom before, during, and after fluoroquinolone treatment.

摘要: Five commercial broiler flocks were treated with a fluoroquinolone for clinically relevant infection. Fresh feces from individual chickens and environmental samples cultured campylobacters before, during, weekly posttreatment until slaughter. Both Campylobacter jejuni C. coli isolated during all treatment phases. An increased proportion of quinolone-resistant strains was seen treatment, these persisted posttreatment. One isolate each species, serotype, phage type sample at phases examined its phenotype mechanism resistance. Two resistant phenotypes isolated: Nalr Cipr Cips. The majority (269 290) fluoroquinolone-resistant isolates, whether they or coli, had mutation in gyrA that resulted the substitution Thr-86→Ile. other mutations detected Thr-86→Ala (n = 17) Asp-90→Asn 10). genotypic variation, based on silent identified by denaturing high-performance liquid chromatography pattern DNA sequencing, used to supplement typing data provided evidence both spread preexisting selection spontaneous mutants flocks. Multidrug resistance significantly (P < 0.01) associated ciprofloxacin. Twenty-five percent (73 ciprofloxacin-resistant isolates but only 13% (24 179) susceptible three more unrelated antimicrobial agents. In conclusion, chicken high numbers following therapy veterinary fluoroquinolone. Most GyrA Resistant some up point slaughter, which may have consequences public health.