A DNA-based molecular probe for optically reporting cellular traction forces

作者: Brandon L Blakely , Christoph E Dumelin , Britta Trappmann , Lynn M McGregor , Colin K Choi

DOI: 10.1038/NMETH.3145

关键词:

摘要: We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations elastic substrates. TPs consist DNA hairpins conjugated fluorophore-quencher pairs unfold fluoresce when subjected specific forces. applied reveal cellular are heterogeneous within focal adhesions localized at their distal edges.

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