T cell clone producing an IL 1-like activity after stimulation by antigen-presenting B cells.

摘要: We have analyzed the involvement of IL 1 in a cellular interaction during which murine B cells serve as antigen-presenting for T cell clone (D10.G4.1). This was chosen study because it is highly dependent on exogenous concanavalin A (or anti-receptor antibody)-induced proliferation. observed that presented either self I-Ak plus nominal antigen or allogeneic I-Ab, and consequently induced proliferation this without any requirement 1. After an overnight co-culture with allogenic cells, we detected 1-like activity lysates mixture, but were unable to detect similar culture medium. MHC-restricted manner. determined intracellular derived from D10.G4.1-responding rather than cells. Moreover, after variety stimuli including LPS, anti-Ig, activated supernatants, combinations these stimuli. Hybridization cytoplasmic RNA oligonucleotide probe human 1-alpha confirmed source biological activity. The factor mixture lysate showed two broad peaks gel filtration, one 10,000 20,000 dalton range second 35,000 45,000 range. first description produced by may introduce new element early events leading activation