Expansion of the Ki-67 proliferative compartment correlates with degree of Dysplasia in Barrett's esophagus

摘要: Background. Barrett's esophagus is a histologically defined premalignant lesion of the in which normal squamous epithelium replaced by intestinalized columnar epithelium. In multistep progression from to fully developed carcinoma, accelerated proliferation may indicate or precede genomic instability and, therefore, be an important factor pathogenesis and/or prediction malignant transformation. Ki-67 nuclear antigen expressed proliferating cells, (G1, S, G2, and M phases) but not resting cells (G0 phase). This study was undertaken determine if expression correlates with degree dysplasia can help differentiate those patients without dysplasia. Methods. The fraction 87 paraffin embedded esophageal biopsies 43 antibody (MIB-1) analyzed using immunohistochemistry. Using computerized index program (QNA v2.54, Becton Dickinson Cellular Imaging Systems, Inc., Elmhurst, IL), score derived for luminal surface, upper crypt, lower underlying glandular zone columnar-lined esophagus. Results. Significant differences scores were noted each among different histologic categories: gastric ([NG] n = 17); ([ND] low grade ([LG] 21); high ([HG] 14); adenocarcinoma ([CA] 5). pattern associated strongly category. percentage positive nuclei mucosal statistically separated (P < 0.001). dysplastic tissues, found predominantly on surface crypt zones, whereas dysplasia, majority zone. number component also significantly when compared tissues. 0.001) Staining patterns indefinite H & E staining into several distinct (five LG, seven ND, one NG) six had more consistent that dysplasia. Conclusion. correlated findings represent additional parameter differentiating dysplasia. Cancer 1995;75:423–9.