Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease.
作者： J.-F. Ma , Y. Huang , S.-D. Chen , G. Halliday
DOI: 10.1111/J.1365-2990.2010.01067.X
关键词:
摘要: J.-F. Ma, Y. Huang, S.-D. Chen and G. Halliday (2010) Neuropathology Applied Neurobiology36, 312–319 Immunohistochemical evidence for macroautophagy in neurones endothelial cells Alzheimer's disease Aim: To determine the pathological structures associated with disease (AD) any relationship to progression. Methods: Immunohistochemistry using antibodies beclin-1, Atg5 Atg12, early markers LC3, mammalian homologue of later marker Atg8, were localized formalin-fixed, paraffin-embedded medial temporal lobe sections AD cases at variable neuritic stages. Double immunofluorescence labelling was used co-localize these Aβ phospho-tau (AT8) correlations performed Spearman rank tests. Results: Atg12 immunoreactivity either dispersed soma dendrites or concentrated tau-immunoreactive dystrophic neurites some neurofibrillary tangles. Fewer Atg12-immunopositive observed longer durations. Atg12-immunoreactive found spatially Aβ-positive plaques, more capillary higher stage. These findings confirmed by other autophagy LC3. Conclusion: The data confirm that occurs undergoing degeneration AD, identified close proximity changes
sci-hub.se 参考文章(30)
Ralph A. Nixon, Jerzy Wegiel, Asok Kumar, Wai Haung Yu, Corrinne Peterhoff, Anne Cataldo, Ana Maria Cuervo, Extensive involvement of autophagy in Alzheimer disease: an immuno-electron microscopy study. Journal of Neuropathology and Experimental Neurology. ,vol. 64, pp. 113- 122 ,(2005) , 10.1093/JNEN/64.2.113
Anne M. Cataldo, Deborah J. Hamilton, Jody L. Barnett, Peter A. Paskevich, Ralph A. Nixon, Abnormalities of the Endosomal-Lysomal System in Alzheimer’s disease Springer, Boston, MA. pp. 271- 280 ,(1996) , 10.1007/978-1-4613-0335-0_34
The endosomal-lysosomal system of neurons in Alzheimer's disease pathogenesis: a review. Neurochemical Research. ,vol. 25, pp. 1161- 1172 ,(2000) , 10.1023/A:1007675508413
Francisco A. Luque, Stephen L. Jaffe, Chapter 8 The Molecular and Cellular Pathogenesis of Dementia of the Alzheimer's Type: An Overview International Review of Neurobiology. ,vol. 84, pp. 151- 165 ,(2009) , 10.1016/S0074-7742(09)00408-5
Daijun Ling, Paul M. Salvaterra, A central role for autophagy in Alzheimer type neurodegeneration Autophagy. ,vol. 5, pp. 738- 740 ,(2009) , 10.4161/AUTO.5.5.8626
Yi Wen, Shaohua Yang, Ran Liu, James W. Simpkins, Transient cerebral ischemia induces site-specific hyperphosphorylation of tau protein. Brain Research. ,vol. 1022, pp. 30- 38 ,(2004) , 10.1016/J.BRAINRES.2004.05.106
Noboru Mizushima, Yoshinori Ohsumi, Tamotsu Yoshimori, Autophagosome formation in mammalian cells. Cell Structure and Function. ,vol. 27, pp. 421- 429 ,(2002) , 10.1247/CSF.27.421
Noboru Mizushima, Aβ generation in autophagic vacuoles Journal of Cell Biology. ,vol. 171, pp. 15- 17 ,(2005) , 10.1083/JCB.200508097
James C. Vickers, Anna E. King, Adele Woodhouse, Matthew T. Kirkcaldie, Jerome A. Staal, Graeme H. McCormack, Catherine A. Blizzard, Ruth E.J. Musgrove, Stanislaw Mitew, Yao Liu, Jyoti A. Chuckowree, Olivier Bibari, Tracey C. Dickson, Axonopathy and cytoskeletal disruption in degenerative diseases of the central nervous system. Brain Research Bulletin. ,vol. 80, pp. 217- 223 ,(2009) , 10.1016/J.BRAINRESBULL.2009.08.004
Masaaki Komatsu, Satoshi Waguri, Tomoki Chiba, Shigeo Murata, Jun-ichi Iwata, Isei Tanida, Takashi Ueno, Masato Koike, Yasuo Uchiyama, Eiki Kominami, Keiji Tanaka, Loss of autophagy in the central nervous system causes neurodegeneration in mice Nature. ,vol. 441, pp. 880- 884 ,(2006) , 10.1038/NATURE04723