Changes in cell cycle status and expression of p34cdc2 kinase during potato tuber meristem dormancy

摘要: Potato (Solanum tuberosum L. cv. Russet Burbank) tubers undergo a period of endodormancy that is characterized by cell division arrest. At the time harvest, used in this study were completely endodormant (i.e. 0% sprouting). After 120 days storage at 3°C, transferred to 20°C had begun exit and exhibited ca 50% sprouting. 223 3°C storage, nondormant 100% Based on flow cytometry, about 70% nuclei isolated from meristems are arrested G 1 /G 0 phase cycle. Storage did not alter cycle position nor transfer 3 for 7 prior analysis unless been stored least days. cold (3°C) showed sprout growth excess 5 mm an increase percentage 2 Uptake incorporation H-thymidine into DNA was low all up until postharvest. time, only incubated incorporation. This coincided with visible growth, demonstrating shifts tuber relate directly breakage per se. Using degenerate primers, portion p34 cdc2 homolog amplified RNA log-phase potato suspension culture cells polymerase chain reaction. Northem probe demonstrated mRNA levels two homologs present throughout period. Immunoblot four proteins containing PSTAIRE epitope cdc2-like) reduced increased reactivated shift kinetics based cytometry These results indicate temporal competence induced dormancy accompanied alterations level homologues but correlated change PSTAIRE-containing proteins. suggests during regulated indirectly posttranscriptional regulation genes controlling