Changes of faecal microbiota in patients with Crohn's disease treated with an elemental diet and total parenteral nutrition

摘要: Abstract Background Intestinal microbiota contributes to the pathogenesis of Crohn's disease. Elemental diet and total parenteral nutrition are effective therapies for disease; however, changes as a result both treatments have not been fully elucidated. Aim To elucidate faecal in disease patients treated with elemental nutrition. Methods Stool samples were collected from 33 active 17 healthy subjects, recollected after (8 patients) (9 patients). Terminal restriction fragment length polymorphism analysis bacterial 16srDNA was performed evaluate whole microbiota. Specific quantitative PCR then used determine populations predominant groups. Results In patients, number terminal fragments, which reflects species, significantly lower. Populations bacteria Bifidobacterium lower ratio Enterococcus higher. The fragments decreased nutrition, but diet. Population Bacteroides fragilis diet, while population increased Conclusion Faecal markedly different subjects. Species diversity reduced by