Role of blood components in ocular silicone oil emulsification. Studies on an in vitro model.

摘要: PURPOSE To develop an in vitro model for silicone oil emulsification and to explore the blood components involved this process. METHODS The capacity of various support (1000 CS) was studied by applying 0.5 ml on top saline containing components. Each tube sonicated 150 seconds centrifuged at 5000 g 20 minutes. Three phases were noted tube: At clear oil, middle emulsified bottom aqueous solution. tubes photographed, percentage phase length (middle) total three calculated from projected image each tube. RESULTS Emulsified plasma or serum initiated after 100 sonication quickly reached maximum (approximately 80%) 120 seconds. size these droplets prepared 0.0467 +/- 0.028 mm, closely resembling that observed samples removed a patient's anterior chamber (0.038 0.018 mm). Under conditions, presence whole cells occurred only concentration micrograms protein/ml; red cell membranes, it 60 protein/ml. Lipoprotein-deficient failed emulsification; however, high-density lipoprotein low-density supported Purified lipoprotein-apolipoproteins emulsification. addition phosphatidylcholine further enhanced process, but alone CONCLUSIONS A simple fast study factors affecting developed. Using model, lipoproteins, purified HDL-apolipoproteins Lipids did not, they had enhance apolipoprotein-supported