Plasma Membrane Organization of Epidermal Growth Factor Receptor in Resting and Ligand-Bound States.

摘要: The spatial arrangement of the epidermal growth factor receptor (EGFR) on cellular plasma membrane is one prime factors that control its downstream signaling pathways and related functions. However, molecular organization, which spans scale from nanometers to micrometer-size clusters, has not been resolved in detail, mainly due a lack techniques with required spatiotemporal resolution. Therefore, we used imaging total internal reflection-fluorescence correlation spectroscopy investigate EGFR dynamics live CHO-K1 membranes resting ligand-bound states. In combination fluorescence diffusion law, this provides information subresolution organization cell membranes. We found overall sensitive both cholesterol actin cytoskeleton. state partly trapped cholesterol-containing domains, whereas another fraction exhibits independent trapping membrane. Disruption cytoskeleton leads broader range coefficients reduction hop diffusion. dose-dependent behavior. At 10 ng/mL EGF endocytosed recycled membrane, do change significantly. 100 forms are subsequently internalized, outside clusters diffusivity increases remains unchanged. After disruption domains or cytoskeleton, induces microscopic endocytosis inhibited.