Differential Expression Profiles of Long Noncoding RNA and mRNA of Osteogenically Differentiated Mesenchymal Stem Cells in Ankylosing Spondylitis

摘要: Objective We previously demonstrated that mesenchymal stem cells (MSC) from patients with ankylosing spondylitis (AS; ASMSC) have a greater osteogenic differentiation capacity than MSC healthy donors (HDMSC) and this difference underlies the pathogenesis of pathological osteogenesis in AS. Here we compared expression levels long noncoding RNA (lncRNA) mRNA between osteogenically differentiated ASMSC HDMSC explored precise mechanism underlying abnormal ASMSC. Methods were induced medium for 10 days. Microarray analyses then performed to identify lncRNA differentially expressed ASMSC, which subjected bioinformatics analysis confirmed by quantitative real-time PCR (qRT-PCR) assays. In addition, coding-non-coding gene co-expression (CNC) networks constructed examine relationships patterns. Results A total 520 665 HDMSC. Bioinformatics revealed 64 signaling pathways significant differences, including transforming growth factor-β signaling. qRT-PCR assays reliability microarray data. The CNC network indicated 4 lncRNA, lnc-ZNF354A-1, lnc-LIN54-1, lnc-FRG2C-3, lnc-USP50-2 may be involved Conclusion Our study characterized differential profiles identified participate These results provide insight into