CRNDE affects the malignant biological characteristics of human glioma stem cells by negatively regulating miR-186

摘要: // Jian Zheng 1 , Xiao-dong Li Ping Wang 2, 3 Xiao-bai Liu Yi-xue Xue Yi Hu Zhen Zhi-qing Zhen-hua 4 Yun-hui Department of Neurosurgery, Shengjing Hospital China Medical University, Shenyang 110004, 2 Neurobiology, College Basic Medicine, 110001, Institute Pathology and Pathophysiology, Physiology, Correspondence to: Liu, e-mail: liuyh_cmuns@163.com Keywords: long non-coding RNAs, CRNDE, microRNAs, miR-186, glioma stem cells Received: January 28, 2015      Accepted: July 02, Published: 13, 2015 ABSTRACT The RNA Colorectal neoplasia differentially expressed (CRNDE) is a novel gene that activated early in colorectal neoplasia, but it also up-regulated many other solid tumors. Herein, the function underlying mechanism CRNDE regulating (GSCs) were investigated. We found expression was while miR-186 down-regulated GSCs. Overexpression could promote cellular proliferation, migration, invasion inhibit apoptosis exerted functions inhibiting migration GSCs promoting apoptosis. And decreased levels XIAP PAK7 by binding to negatively it. In addition, binded 3′UTR region, decrease them, thus downstream target proteins such as caspase 3, BAD, cyclin D1 MARK2. vivo effect showed tumor formation rate minimum tumor-bearing nude mice with knockdown overexpression miR-186. conclusion, played an oncogenic role through negative regulation Both be regarded potential targets therapy.