Long non-coding RNA CCAT1 promotes glioma cell proliferation via inhibiting microRNA-410
作者： Zhao-Hui Wang , Xia-Qing Guo , Qi-Shun Zhang , Jian-Lei Zhang , Yan-Li Duan
DOI: 10.1016/J.BBRC.2016.10.047
关键词:
摘要: Abstract Background and aim Long non-coding RNAs have been confirmed to play a critical role in various cancers. In the present study, effect of long RNA (lncRNA) CCAT1 on glioma cell proliferation its potential mechanism were investigated. Methods results Real-time PCR showed that lncRNA-CCAT1 expression was significantly upregulated cancer tissues lines compared with controls. After inhibiting line U251 siRNA-CCAT1 (si-CCAT1), viability colony formation decreased, cycle arrested G1 phase, apoptosis increased. As reported bioinformatics software starbase2.0, total 22 microRNAs potentially targeted by CCAT1. It miR-410 altered most si-CCAT1. up-regulating U251 cells, level decreased. Luciferase reporter assay miR-410. Correlation analysis negatively related tissues. addition, down-regulation reversed inhibitory si-CCAT1 proliferation. Conclusion These data demonstrated promoted via miR-410, providing new insight about pathogenesis
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