Magnetofection Based on Superparamagnetic Iron Oxide Nanoparticles Weakens Glioma Stem Cell Proliferation and Invasion by Mediating High Expression of MicroRNA-374a.

摘要: Glioma stem cells belong to a special subpopulation of glioma that are characterized by strong proliferation, invasion and drug resistance capabilities. Magnetic nanoparticles nanoscale biological materials with magnetic properties. In this study, CD133(+) primary were isolated from patients cultured. Then, used mediate the transfection expression microRNA-374a overexpression plasmid in cells. Transmission electron microscopy detected presence significant nanoparticle substances within after transfection. The qRT-PCR Northern blot results showed could be achieve into efficient mature microRNA-374a. MTT flow cytometry proliferation inhibition rate was significantly higher group than microRNA-mut group; additionally, former presented cell cycle arrest. Transwell experiments confirmed reduce invasiveness Moreover, high mediated effectively reduced tumourigenicity nude mice. luciferase assays revealed fragments bind 3'UTR Neuritin (NRN1), thereby interfering mRNA expression. Western blotting genes such as NRN1, CCND1, CDK4 Ki67 Thus, can efficiently microRNA plasmids mammalian silence NRN1 expression, inhibiting vivo human