Modulation of CREB activity by the Rho GTPase regulates cell and organism size during mouse embryonic development

摘要: Rho GTPases regulate several aspects of tissue morphogenesis during animal development. We found that mice lacking the Rho-inhibitory protein, p190-B RhoGAP, are 30% reduced in size and exhibit developmental defects strikingly similar to those seen CREB transcription factor. In RhoGAP-deficient mice, phosphorylation is substantially embryonic tissues. Embryo-derived cells contain abnormally high levels active size, activation upon exposure insulin or IGF-1. The cell defect rescued by expression constitutively activated CREB, wild-type cells, dominant-negative results size. Together, these suggest activity GTPase modulates a signal from insulin/IGFs determines embryogenesis.