Detection of apple chlorotic leaf spot virus using a 5' nuclease assay with a fluorescent 3' minor groove binder-DNA probe.
作者： Michel A. Salmon , Marina Vendrame , Jean Kummert , Philippe Lepoivre
DOI: 10.1016/S0166-0934(02)00035-6
关键词:
摘要: The development of a real-time 5' nuclease RT-PCR assay for the detection apple chlorotic leaf spot virus (ACLSV) from infected plant material is described. A short fluorogenic 3' minor groove binder-DNA hydrolysis probe was used to circumvent genome variability between isolates and target conserved sequence. covalent attachment binder moiety at end increased probe/target duplex stability raised melting temperature range suitable analysis. method rapid, sensitive takes place within single tube without post-PCR handling amplification products.
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