A one‐step multiplex RT‐PCR assay for simultaneous detection of four viruses that infect peach

摘要: UNLABELLED A multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect peach, namely Apple chlorotic leaf spot virus (ACLSV), Cherry green ring mottle (CGRMV), Prunus necrotic ringspot (PNRSV) Apricot pseudo-chlorotic (APCLSV). In this study, pairs primers, one specific for each virus, were designed; corresponding PCR products 632, 439, 346 282 bp in length ACLSV, CGRMV, PNRSV APCLSV, respectively, fragments could be distinguished clearly by agarose gel electrophoresis. The sensitivity specificity method tested using individual RT-PCR enzyme-linked immunosorbent (ELISA), identity amplification also confirmed DNA sequencing. results ELISA, along with batch samples collected from peach orchards, revealed rapid simple technique is an effective way identify simultaneously. SIGNIFICANCE AND IMPACT OF THE STUDY mRT-PCR described study infected reliable sensitive. contrast conventional uniplex RT-PCR, more efficient, reducing costs, time handling when testing large numbers samples. This useful large-scale surveys peach.