Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
作者： Hongsup Kim , Seung-In Yi , Jung Sik Eom , Jeongran Park , Som Gyeol Lee
DOI: 10.5423/RPD.2018.24.3.233
关键词:
摘要: A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of recently reported viruses, Cherry virus (CVA), Little cherry 1 (LChV-1), and 2 (LChV-2), in plants Korea. Eight sets primers were designed each their specificity tested by using various combinations mixed primer sets. From sets, one combination selected further evaluated to estimate optimum temperature limits mRT-PCR. newly mRT-PCR also 20 samples collected field. This may be a useful tool field surveys diseases rapid these three viruses plants.
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