摘要: The kinetics of actin polymerization were analyzed by taking into account nucleation, elongation, and spontaneous fragmentation filaments. Polymerization curves measured in the presence potassium (40 mM) found to be good agreement with calculated for assumption that nucleation elongation but no reactions occur. calcium (1.8 or magnesium (0.6 mM MgCl2 0.5 EGTA) could only stimulated when was assumed occur addition elongation. experiments reported this study even absence ultrasonication shear forces filaments may break spontaneously extent depends strongly on experimental conditions. Spontaneous changes shape significantly. When takes place, a relatively long lag phase is observed followed increasing rate reach final constant value quickly. On other hand, are formed exclusively approach slowly after short initial phase.
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sci-hub.se 参考文章(16)
Edward F. Casassa, Light Scattering from Very Long Rod‐Like Particles and an Application to Polymerized Fibrinogen Journal of Chemical Physics. ,vol. 23, pp. 596- 597 ,(1955) , 10.1063/1.1742048
Michael K. Rees, Michael Young, Studies on the Isolation and Molecular Properties of Homogeneous Globular Actin: EVIDENCE FOR A SINGLE POLYPEPTIDE CHAIN STRUCTURE Journal of Biological Chemistry. ,vol. 242, pp. 4449- 4458 ,(1967) , 10.1016/S0021-9258(18)99559-1
MASARU KAWAMURA, KOSCAK MARUYAMA, Electron microscopic particle length of F-actin polymerized in vitro. Journal of Biochemistry. ,vol. 67, pp. 437- 457 ,(1970) , 10.1093/OXFORDJOURNALS.JBCHEM.A129267
Hiroshi KONDO, Shin'ichi ISHIWATA, Uni-directional growth of F-actin. Journal of Biochemistry. ,vol. 79, pp. 159- 171 ,(1976) , 10.1093/OXFORDJOURNALS.JBCHEM.A131043
S.L. Brenner, E.D. Korn, Substoichiometric concentrations of cytochalasin D inhibit actin polymerization. Additional evidence for an F-actin treadmill. Journal of Biological Chemistry. ,vol. 254, pp. 9982- 9985 ,(1979) , 10.1016/S0021-9258(19)86660-7
Fumio Oosawa, Michiki Kasai, A theory of linear and helical aggregations of macromolecules Journal of Molecular Biology. ,vol. 4, pp. 10- 21 ,(1962) , 10.1016/S0022-2836(62)80112-0
C. J. Lusty, H. Fasold, Characterization of sulfhydryl groups of actin. Biochemistry. ,vol. 8, pp. 2933- 2939 ,(1969) , 10.1021/BI00835A036
Yasuo Nakaoka, Michiki Kasai, Behaviour of sonicated actin polymers: Adenosine triphosphate splitting and polymerization Journal of Molecular Biology. ,vol. 44, pp. 319- 332 ,(1969) , 10.1016/0022-2836(69)90178-8
Terence P. Walsh, Albrecht Wegner, Effect of the state of oxidation of cysteine 190 of tropomyosin on the assembly of the actin-tropomyosin complex. Biochimica et Biophysica Acta. ,vol. 626, pp. 79- 87 ,(1980) , 10.1016/0005-2795(80)90199-3
Albrecht Wegner, Juergen Engel, Kinetics of the cooperative association of actin to actin filament Biophysical Chemistry. ,vol. 3, pp. 215- 225 ,(1975) , 10.1016/0301-4622(75)80013-5