Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization.
作者： Yukiko Shinozaki , Tomotake Morita , Xiao-hong Cao , Shigenobu Yoshida , Motoo Koitabashi
DOI: 10.1007/S00253-012-4188-8
关键词:
摘要: Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and succinate-co-adipate) (PBSA). An enzyme named PaE was isolated the gene encoding cloned from strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence contains 198 acids with predicted molecular weight 20,362.41. High identity observed between this that cutinase-like enzymes (CLEs) (61–68 %); therefore, PaCLE1. specific against emulsified PBSA 54.8 ± 6.3 U/mg. In addition to BPs, degraded solid PBS, PBSA, poly(e-caprolactone), poly(lactic acid).
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sci-hub.se 参考文章(28)
Y. Oda, A. Yonetsu, T. Urakami, K. Tonomura, Degradation of Polylactide by Commercial Proteases Journal of Polymers and The Environment. ,vol. 8, pp. 29- 32 ,(2000) , 10.1023/A:1010120128048
Akira Hoshino, Yasuyuki Isono, Degradation of aliphatic polyester films by commercially available lipases with special reference to rapid and complete degradation of poly(L-lactide) film by lipase PL derived from Alcaligenes sp. Biodegradation. ,vol. 13, pp. 141- 147 ,(2002) , 10.1023/A:1020450326301
H Pranamuda, Y Tokiwa, H Tanaka, Polylactide Degradation by an Amycolatopsis sp. Applied and Environmental Microbiology. ,vol. 63, pp. 1637- 1640 ,(1997) , 10.1128/AEM.63.4.1637-1640.1997
Tadahisa Iwata, Yoshiharu Doi, Morphology and Enzymatic Degradation of Poly(l-lactic acid) Single Crystals Macromolecules. ,vol. 31, pp. 2461- 2467 ,(1998) , 10.1021/MA980008H
Mark E. Schmitt, Timothy A. Brown, Bernard L. Trumpower, A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae Nucleic Acids Research. ,vol. 18, pp. 3091- 3092 ,(1990) , 10.1093/NAR/18.10.3091
Jörg Kämper, Regine Kahmann, Michael Bölker, Li-Jun Ma, Thomas Brefort, Barry J Saville, Flora Banuett, James W Kronstad, Scott E Gold, Olaf Müller, Michael H Perlin, Han AB Wösten, Ronald De Vries, José Ruiz-Herrera, Cristina G Reynaga-Peña, Karen Snetselaar, Michael McCann, José Pérez-Martín, Michael Feldbrügge, Christoph W Basse, Gero Steinberg, Jose I Ibeas, William Holloman, Plinio Guzman, Mark Farman, Jason E Stajich, Rafael Sentandreu, Juan M González-Prieto, John C Kennell, Lazaro Molina, Jan Schirawski, Artemio Mendoza-Mendoza, Doris Greilinger, Karin Münch, Nicole Rössel, Mario Scherer, Miroslav Vraneš, Oliver Ladendorf, Volker Vincon, Uta Fuchs, Björn Sandrock, Shaowu Meng, Eric CH Ho, Matt J Cahill, Kylie J Boyce, Jana Klose, Steven J Klosterman, Heine J Deelstra, Lucila Ortiz-Castellanos, Weixi Li, Patricia Sanchez-Alonso, Peter H Schreier, Isolde Häuser-Hahn, Martin Vaupel, Edda Koopmann, Gabi Friedrich, Hartmut Voss, Thomas Schlüter, Jonathan Margolis, Darren Platt, Candace Swimmer, Andreas Gnirke, Feng Chen, Valentina Vysotskaia, Gertrud Mannhaupt, Ulrich Güldener, Martin Münsterkötter, Dirk Haase, Matthias Oesterheld, Hans-Werner Mewes, Evan W Mauceli, David DeCaprio, Claire M Wade, Jonathan Butler, Sarah Young, David B Jaffe, Sarah Calvo, Chad Nusbaum, James Galagan, Bruce W Birren, None, Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis Nature. ,vol. 444, pp. 97- 101 ,(2006) , 10.1038/NATURE05248
Fan Li, Sha Wang, Weifeng Liu, Guanjun Chen, Purification and characterization of poly(L-lactic acid)-degrading enzymes from Amycolatopsis orientalis ssp. orientalis. Fems Microbiology Letters. ,vol. 282, pp. 52- 58 ,(2008) , 10.1111/J.1574-6968.2008.01109.X
Richard A Gross, Bhanu Kalra, Biodegradable Polymers for the Environment Science. ,vol. 297, pp. 803- 807 ,(2002) , 10.1126/SCIENCE.297.5582.803
Fusako Kawai, Kosuke Nakadai, Emiko Nishioka, Hajime Nakajima, Hitomi Ohara, Kazuo Masaki, Haruyuki Iefuji, Different enantioselectivity of two types of poly(lactic acid) depolymerases toward poly(l-lactic acid) and poly(d-lactic acid) Polymer Degradation and Stability. ,vol. 96, pp. 1342- 1348 ,(2011) , 10.1016/J.POLYMDEGRADSTAB.2011.03.022
N.R Kamini, T Fujii, T Kurosu, H Iefuji, Production, purification and characterization of an extracellular lipase from the yeast, Cryptococcus sp. S-2 Process Biochemistry. ,vol. 36, pp. 317- 324 ,(2000) , 10.1016/S0032-9592(00)00228-4