Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome information

摘要: Amino acid assimilation is crucial for bacteria and this particularly true Lactic Acid Bacteria (LAB) that are generally auxotroph amino acids. The global response of the LAB model Lactococcus lactis ssp. was characterized during progressive isoleucine starvation in batch culture using a chemically defined medium which concentration fixed so as to become sole limiting nutriment. Dynamic analyses were performed transcriptomic proteomic approaches results analysed conjointly with fermentation kinetic data. first deduced from analysis corroborated by results. It occurred progressively could be divided into three major mechanisms: (i) down-regulation processes linked bacterial growth catabolism (transcription, translation, carbon metabolism transport, pyrimidine fatty metabolism), (ii) specific positive related nutrient (activation pathways or nitrogen leading supply) (iii) an unexpected oxidative stress (positive regulation aerobic metabolism, electron thioredoxin pyruvate dehydrogenase). involvement various regulatory mechanisms adaptation on basis data comparisons. regulator CodY seemed specifically dedicated supply. Other regulations massively rate stringent response. This integrative biology approach provided overview metabolic involved their regulations. has extended significantly physiological understanding L. lactis. can generalised other conditions will contribute identification biological complex networks micro-organisms.