Neuromedin U can exert colon-specific, enteric nerve-mediated prokinetic activity, via a pathway involving NMU1 receptor activation
作者： N B Dass , A K Bassil , V J North-Laidler , R Morrow , E Aziz
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摘要: Background and purpose: The neuromedin U (NMU) receptors, NMU1 NMU2, are expressed in the gut but their functions unclear. This study explores role of NMU gastrointestinal motility. Experimental approach: The effects were examined forestomach colon isolated from NMU2R wild-type NMU2R-/- (knockout) mice, looking for changes muscle tension nerve-mediated responses evoked by electrical field stimulation (EFS), models peristalsis mouse faecal pellet transit guinea-pig colon. Key results: In forestomach, (1 nM-10 μM) concentration-dependently induced contraction, presence tetrodotoxin atropine, preparations both mice (pEC50: 7.9, 7.6, Emax: 0.26, 0.20g tension, respectively, n=8 each concentration). The same concentrations had no consistent on to EFS (n=8). In colon, (0.1 nM-1 significant effect baseline (n=8), potentiated EFS-evoked contractions pEC50: 8.1, 7.8, 24%, 21%, n=6-11. (0.01 nM-0.1 μM, n=5-7) decreased interval between waves 8.8) increased rate at which a moved along colon. Conclusions implications: These results demonstrate that exerts colon-specific, nerve-mediated, prokinetic activity, via pathway involving activation receptors. suggests this receptor may represent molecular target treatment intestinal motility disorders. British Journal Pharmacology (2007) 150, 502–508. doi:10.1038/sj.bjp.0707004
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