Gender differences in diet-induced steatotic disease in Cyp2b-null mice.

摘要: Nonalcoholic fatty liver disease (NAFLD) is the most common disease; however, progression to nonalcoholic steatohepatitis (NASH) associated with adverse outcomes. CYP2B metabolizes multiple xeno- and endobiotics, male Cyp2b-null mice are diet-induced obese (DIO) increased NAFLD. However, DIO study was not performed long enough assess NASH. Therefore, role of Cyp2b in from NAFLD NASH, we treated wildtype (WT) a normal diet (ND) or choline-deficient, L-amino acid-defined high fat (CDAHFD) for 8 weeks determined metabolic molecular changes. CDAHFD-fed WT female gained more weight had greater white adipose tissue mass than their counterparts; males experienced loss regardless genotype. Serum biomarkers injury both mice; however females exhibited significantly lower serum ALT, AST, ASP concentrations compared mice, indicating were protected injury. In genders, hierarchical clustering RNA-seq data demonstrates several gene ontologies responded differently (lipid metabolism > fibrosis inflammation). Oil Red O staining direct triglycerides measurements confirmed that showed equivocal changes transcriptomic markers suggesting less inflammation due glucocorticoid-mediated repression immune responses. contrast females, higher triglyceride levels. Results indicate while susceptible NAFLD, few significant NASH development. This confirms development does necessarily lead progressive Furthermore, it indicates differs based on gender.