Early Pregnancy Maternal Blood DNA Methylation in Repeat Pregnancies and Change in Gestational Diabetes Mellitus Status—A Pilot Study

摘要: Repeat pregnancies with different perinatal outcomes minimize underlying maternal genetic diversity and provide unique opportunities to investigate nongenetic risk factors epigenetic mechanisms of pregnancy complications. We investigated gestational diabetes mellitus (GDM)-related differential DNA methylation in early peripheral blood samples collected from women who had a change GDM status repeat pregnancies. Six study participants were randomly selected among 2 consecutive pregnancies, only 1 which was complicated by (case pregnancy) the other not (control pregnancy). Epigenome-wide profiled using Illumina HumanMethylation 27 BeadChips. Differential Identification Mixture Ensemble false discovery rate (<10%) cutoffs used identify differentially methylated targets between each participant. Overall, target sites, 17 hypomethylated (fold [FC] range: 0.77-0.99) 10 hypermethylated (FC 1.01-1.09), control 5 or more participants. Novel genes related identified (such as NDUFC1, HAPLN3, HHLA3, RHOG) sites SEP11, ZAR1, DDR). Genes participated cell morphology, cellular assembly, organization, compromise, cycle. Our findings support differences status. Similar, larger, studies can enhance biomarker mechanistic GDM.