Immunity in cutaneous leishmaniasis of the guinea-pig.

摘要: This paper describes the course of infection and development immunity in guinea-pigs after intradermal inoculation Leishmania enriettii, use vivo vitro techniques to characterize immunological response artificial immunization. Inoculation 106 amastigotes into ear produced a nodule which ulcerated 2–3 weeks healed 8–16 weeks. 8% animals developed cutaneous metastases with original lesions. Histology primary lesions showed epidermal necrosis overlying mass parasitized macrophages which, 4–6 weeks, became surrounded infiltrated by lymphocytes. Histological changes draining lymph node began 3 days proceeded for 6 weeks; both germinal centres paracortical areas were hyperplastic medulla contained many plasma cells. Superinfection an `isophasic' lesion, but reinfection healing elicited only delayed hypersensitivity response. Artificial immunization soluble insoluble antigenic extracts L. enriettii Freund's complete adjuvant partially protected against infection; other leishmanial species failed protect. Immunological paralysis, attempted intravenous injections antigen, increased severity subsequent infection. Both accompanied could be transferred passively lymphoid Cell-mediated was studied ability antigens transform lymphocytes, inhibit macrophage migration, induce production lymphokine factors from lymphocytes sensitized animals. A target cell system devised destroyed monolayers macrophages. Cross reactivity mycobacterial shown skin tests destruction, not transfer or culture systems. The phagocytic activity peritoneal recovered homologous heterologous Leishmania; growth ingested organisms however reduced. Circulating antibodies demonstrated passive anaphylaxis, agglutination antigen coated sheep erythrocytes, sera infected convalescent animals, although some active anaphylaxis. However, these immunized also anaphylactic Arthus when tested antigens. The results are taken indicate that cellular mechanisms prominent guinea-pig ways host may eliminate parasite discussed. It is concluded this model provides experimental counterpart human leishmaniasis it suitable analysis role cell-mediated specific resistance intracellular infection.