Lineage-related cytotoxicity and clonogenic profile of 1,4-benzoquinone-exposed hematopoietic stem and progenitor cells.
作者： Paik Wah Chow , Zariyantey Abdul Hamid , Kok Meng Chan , Salmaan Hussain Inayat-Hussain , Nor Fadilah Rajab
DOI: 10.1016/J.TAAP.2015.01.016
关键词:
摘要: Abstract Hematopoietic stem cells (HSCs) and hematopoietic progenitor (HPCs) are sensitive targets for benzene-induced hematotoxicity leukemogenesis. The impact of benzene exposure on the complex microenvironment HSCs HPCs remains elusive. This study aims to investigate mechanism linking targeting using phenotypic clonogenic analyses. Mouse bone marrow (BM) were exposed ex vivo metabolite, 1,4-benzoquinone (1,4-BQ), 24 h. Expression cellular surface antigens HSC (Sca-1), myeloid (Gr-1, CD11b), lymphoid (CD45, CD3e) populations confirmed by flow cytometry. clonogenicity was studied colony-forming unit (CFU) assay multilineage (CFU-GM CFU-GEMM) single-lineage (CFU-E, BFU-E, CFU-G, CFU-M) progenitors. 1,4-BQ demonstrated concentration-dependent cytotoxicity in mouse BM cells. percentage apoptotic increased (p
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sci-hub.se 参考文章(48)
ZB Hu, W Ma, CC Uphoff, K Metge, SM Gignac, HG Drexler, Myeloperoxidase: expression and modulation in a large panel of human leukemia-lymphoma cell lines Blood. ,vol. 82, pp. 1599- 1607 ,(1993) , 10.1182/BLOOD.V82.5.1599.1599
Tu-Lai Yew, Shih-Chieh Hung, Wei-Hua Huang, Der-Chi Yang, Chih-Chien Tsai, Benefits of hypoxic culture on bone marrow multipotent stromal cells American journal of blood research. ,vol. 2, pp. 148- 159 ,(2012)
R Snyder, G Witz, B D Goldstein, The toxicology of benzene. Environmental Health Perspectives. ,vol. 100, pp. 293- 306 ,(1993) , 10.1289/EHP.93100293
J-F Tian, C-H Peng, X-Y Yu, X-J Yang, H-T Yan, Expression and methylation analysis of p15 and p16 in mouse bone marrow cells exposed to 1,4-benzoquinone Human & Experimental Toxicology. ,vol. 31, pp. 718- 725 ,(2012) , 10.1177/0960327111422403
M T Smith, J W Yager, K L Steinmetz, D A Eastmond, Peroxidase-dependent metabolism of benzene's phenolic metabolites and its potential role in benzene toxicity and carcinogenicity Environmental Health Perspectives. ,vol. 82, pp. 23- 29 ,(1989) , 10.1289/EHP.898223
M T Smith, THE MECHANISM OF BENZENE-INDUCED LEUKEMIA : A HYPOTHESIS AND SPECULATIONS ON THE CAUSES OF LEUKEMIA Environmental Health Perspectives. ,vol. 104, pp. 1219- 1225 ,(1996) , 10.1289/EHP.961041219
Veronica C Nwosu, Grace E Kissling, Carol S Trempus, Hayden Honeycutt, John E French, Exposure of Tg.AC transgenic mice to benzene suppresses hematopoietic progenitor cells and alters gene expression in critical signaling pathways. Toxicology and Applied Pharmacology. ,vol. 196, pp. 37- 46 ,(2004) , 10.1016/J.TAAP.2003.11.010
D. Parent-Massin, Relevance of clonogenic assays in hematotoxicology Cell Biology and Toxicology. ,vol. 17, pp. 87- 94 ,(2001) , 10.1023/A:1010906104558
Magdalena L. Circu, Tak Yee Aw, Reactive oxygen species, cellular redox systems, and apoptosis Free Radical Biology and Medicine. ,vol. 48, pp. 749- 762 ,(2010) , 10.1016/J.FREERADBIOMED.2009.12.022
Hiroyuki Shiono, Hong Miao Chen, Tadashi Okada, Yoichiro Ito, Colony-forming cell assay for human hematopoietic progenitor cells harvested by a novel continuous-flow cell separation method Journal of Chromatography A. ,vol. 1151, pp. 153- 157 ,(2007) , 10.1016/J.CHROMA.2007.01.021