Expression and methylation analysis of p15 and p16 in mouse bone marrow cells exposed to 1,4-benzoquinone
作者： J-F Tian , C-H Peng , X-Y Yu , X-J Yang , H-T Yan
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摘要: Benzene is an important industrial chemical. It also environmental pollutant recognized as a human carcinogen. Both prenatal and adult exposures to benzene are associated with the development of leukemia. To understand mechanism benzene-induced epigenetic variations, we investigated expression methylation patterns CpG (phosphodiester bond between cytosine guanine) islands in p15 p16 promoter regions 1,4-benzoquinone (1,4-BQ)-treated primary cultivated C57BL/6J mouse bone marrow cells vitro. The cell toxicity 1,4-BQ was evaluated by viability test, real-time PCR used measure mRNA levels, bisulfite sequencing (BSP) look into patterns. test indicates that exhibited dose-dependent cells. After 24-h exposure at final concentrations 0, 0.1, 1, 10 μmol/L, decreased increase concent...
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