Morphologic assessment of leukocyte-endothelial cell interactions in mesenteric venules subjected to ischemia and reperfusion.

摘要: Intravital microscopic studies of the mesenteric microcirculation have demonstrated that leukocyte adherence and emigration in postcapillary venules are a characteristic feature tissues exposed to ischemia-reperfusion. The objectives this study were determine whether: (1) neutrophils predominant leukocytes adhere emigrate postischemic venules, (2) and/or prerequisite for reperfusion-induced increases venular permeability. Leukocyte kinetics cat (25–35μm diameter) evaluated using both intravital microscopy quantitative morphometry. intestine mesentery 60 min ischemia, followed by reperfusion. Some animals pretreated with monoclonal antibody (MoAb IB4) against adhesion glycoprotein, CD11/CD18. Vessels observed adjacent similar diameter excised processed light (LM) electron (EM). Horseradish peroxidase (HRP), administered intravenously, was used assess vascular permeability EM. By LM, control (nonischemic) is sparsely populated plasma cells, mast leukocytes; 30–50% resident population neutrophils. Ischemia-reperfusion led significant increase number extravascular accounting >80% total cell population. Control ischemic no leakage HRP into interstitium. However, ischemia reperfusion between endothelial cells surrounding interstitium; adherent luminal surface endothelium, transmigrating vessel wall, Animals MoAb IB4 presented same profile as nonischemic controls, or some noted following treated IB4. results indicate that: predominate inhibition does not completely prevent dysfunction associated