Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages

摘要: Containment of Mycobacterium tuberculosis (Mtb) infection requires T cell recognition infected macrophages. Mtb evolved to tolerate, evade, and subvert host immunity. Despite a vigorous sustained CD8+ response during infection, cells make limited contribution protection. Here, we ask whether the ability Mtb-specific restrict growth is related their capacity recognize Mtb-infected We derived lines that recognized immunodominant epitope TB10.44-11 compared them CD4+ Ag85b240-254 or ESAT63-17. While macrophages inhibited in vitro, TB10.4-specific neither nor restricted growth. with Listeria monocytogenes expressing TB10.4. However, over-expression TB10.4 did not confer by cells. Importantly, pulsed irradiated Mtb, indicating can efficiently cross-present protein raising possibility viable bacilli might suppress cross-presentation. polyclonal specific for antigens other than MHC-restricted manner. As elicits dominant poorly recognizes macrophages, propose acts as decoy antigen. Moreover, it appears this overshadows subdominant The may explain why disproportionately small defense selection vaccines has focused on generate responses. establishing vaccine-elicited could be useful criterion preclinical vaccine development.