Development of CD8+ Effector T Cells Is Differentially Regulated by IL-18 and IL-12
作者： Keizo Kohno , Masashi Kurimoto , Tadao Tanimoto , Hakuo Ikegami , Iwao Okamoto
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摘要: We investigated the effects of IL-18 on development CD8 + effector T cells in DBA/2 anti-BDF 1 whole spleen cell MLC and compared results with those IL-12. Addition to resulted a twofold increase CD8/CD4 ratios control cultures when were expanded IL-2-containing medium following MLC. Purified recovered from IL-18-stimulated produced 20- 30-fold more IFN-γ after secondary stimulation C57BL/6 or anti-CD3 mAb, exhibited strong allospecific CTL activity. Neither nor IL-18-supplemented culture supernatants induced type I purified used as responder primary Furthermore, CD4 depletion abrogated IL-18-induced production by cells, suggesting that was dependent. In marked contrast, adding IL-12 decreased 50% suppressed activity regardless concentration, whereas Th1 promoted Moreover, both efficiently These findings show plays an important role generation further suggest functional maturation is differentially regulated
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