Preparation and characterization of three-dimensional nanostructured macroporous bacterial cellulose/agarose scaffold for tissue engineering

摘要: We fabricated a three-dimensional nanostructured macroporous bacterial cellulose scaffold (3D BC scaffold) and cellulose/agarose BC/A). Results of scanning electron microscopy (SEM) mercury intrusion porosimeter showed that both the 3D BC/A have interconnected macropores characterized by nanofibrous pore walls (The diameter dominant pores was about 100 μm ranges from 1,000 μm). also has high surface area (80 ± 5 m2/g) sufficient porosity (88.5 0.4%) compare with (surface area: 92.81 2.02 m2/g; 90.42 0.24%). do support C5.18 cell hBMSC attachment, proliferation evaluated SEM, confocal assay. Furthermore, 3D/ABC enhanced mechanical property (ultimate compressive strength: 26.26 4.6 kPa, Young’s modulus: 39.26 5.72 kPa)) than 3D/BC 7.04 2.34 10.76 3.52 kPa). Overall, had more potential for using as tissue engineering repair applications.