Differentiation of a Fibrin Gel Encapsulated Chondrogenic Cell Line

摘要: Hyaline cartilage has very limited regenerative capacity following damage. Therefore engineered tissue substitutes have been the focus of much research. Our objective was to develop a fibrin-based scaffold as cell delivery vehicle and template for hyaline regeneration, compare its cellular properties against monolayer pellet culture chondrogenic cells. The precursor line, RCJ 3.1C5.18 (C5.18), chosen test system evaluating effect various conditions, including encapsulation, on articular differentiation. C5.18 cells in showed elevated expression collagen II, an marker, but also markers fibrocartilage differentiation (collagen I versican) when cultured with medium compared basic maintenance medium. Pellets were histologically more organized structure than pellets control secreted extracellular matrix that comprised type II little collagen, indicating trend towards hyaline-like cartilage. Moreover, medium, fibrin-encapsulated elaborated containing well aggrecan, which are both components This indicated articular-like fibrin encapsulated results these experiments provide evidence line can be used tool evaluate potential scaffolds engineering.