Variations in matrix composition and GAG fine structure among scaffolds for cartilage tissue engineering

摘要: Summary Objective To compare matrix composition and glycosaminoglycan (GAG) fine structure among five scaffolds commonly used for in vitro chondrocyte culture cartilage tissue engineering. Design Bovine articular chondrocytes were seeded into agarose, alginate, collagen I, fibrin polyglycolic acid (PGA) constructs cultured 20 or 40 days. In addition to construct DNA sulfated GAG (sGAG) contents, the Δ-disaccharide compositions of chondroitin/dermatan sulfate GAGs determined each scaffold group via fluorophore-assisted carbohydrate electrophoresis (FACE). Results Significant differences found cell proliferation extracellular accumulation groups. was observed all types but occurred later (20–40 days) PGA compared other groups (0–20 days). By days, agarose had highest sGAG ratio, while alginate I lowest levels. Quantitative accumulated different also found, with most striking variations unsulfated disulfated Δ-disaccharides. Agarose fraction residues residues, a 6-sulfated/4-sulfated disaccharide ratio similar that native cartilage. Conclusions The similarities proteoglycan suggest material directly indirectly influences metabolism may have an influence on quality engineered