Ecdysone metabolism by soluble enzymes from three species of diptera and its inhibition by the insect growth regulator TH-6040

摘要: Abstract Homogenates of larvae, pupae, and adults house flies ( Musca domestica L.), flesh Sarcophaga bullata Parker), blow Phormia regina (Meigen)) have been examined for enzymes which convert α- β-ecdysone to apolar products. Most the activity was found in soluble fraction from but none fly fractions active. Two seem be involved ecdysone metabolism, one requiring NADPH other functioning without this cofactor. The product latter enzyme is thought 3-dehydro-ecdysone. This further converted 3α-hydroxy isomer by NADPH-requiring enzyme. On feeding insect growth regulator TH-6040 (1-(4-chlorophenyl)-3-(2,6-difluorobenzoyl)-urea) larvae at dietary levels ranging 0.3 10 ppm, producing 3-dehydro reduced 20 82%. It suggested that exerts its effect on pupal-adult ecdysis through inhibition metabolism.