Ecdysone Oxidase, an enzyme from the blowfly Calliphora erythrocephala (Meigen).

摘要: In the blowfly, formation of 3-dehydroecdysone from insect molting hormone ecdysone is catalyzed by an enzyme which carries hydrogen and ecdysterone to oxygen. The therefore called "ecdysone oxidase". Two methods are described for detection oxidase activity, one using a radiolabelled substrate separated product thin-layer chromatography after reaction, other dichloroindophenol, discoloured redox reaction. purified factor 2200 prepupae Calliphora erythrocephala salt precipitation ion exchange chromatography. has Km value 42muM. pH optimum 6.5. temperature lies at 45 degrees C. molecular weight 240000.