Enhanced Epithelial-Mesenchymal Transition-like Phenotype inN-Acetylglucosaminyltransferase V Transgenic Mouse Skin Promotes Wound Healing
作者： Mika Terao , Akiko Ishikawa , Susumu Nakahara , Akihiro Kimura , Arisa Kato
关键词:
摘要: N-Acetylglucosaminyltransferase V (GnT-V) catalyzes the β1,6 branching of N-acetylglucosamine on N-glycans. GnT-V expression is elevated during malignant transformation in various types cancer. However, mechanism by which promotes cancer progression unclear. To characterize biological significance GnT-V, we established transgenic (Tg) mice, regulated a β-actin promoter. No spontaneous was detected any organs Tg mice. up-regulated mouse skin, and cultured keratinocytes derived from these mice showed enhanced migration, associated with changes E-cadherin localization epithelial-mesenchymal transition (EMT). Further, EMT-associated factors snail, twist, N-cadherin were up-regulated, cutaneous wound healing accelerated vivo. We further investigated detailed mechanisms EMT assessing EGF signaling found receptor keratinocytes. These findings indicate that overexpression keratinocyte migration part through signaling.
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